Journal: Biological Research

Article Title: CoCl 2 simulated hypoxia induce cell proliferation and alter the expression pattern of hypoxia associated genes involved in angiogenesis and apoptosis

doi: 10.1186/s40659-019-0221-z

Figure Lengend Snippet: Differential expression pattern of HIF-1α and hypoxia associated genes involved in angiogenesis and apoptosis like VEGF, p53 and BAX in breast cancer cells, where graphical data represents the relative density value after densitometry analysis of band of PCR amplified product. a Depicts the expression pattern of β-actin, HIF-1α and VEGF in MCF-7 cells treated with different concentration of CoCl 2 as compare to control by RT-PCR, similarly b shows expression of β-actin, HIF-1α, VEGF, p53 and BAX genes in MDA-MB-231 cells treated with increasing concentration of CoCl 2 , where it was clearly observed that expression of these genes increases significantly in a dose dependent manner. Error bar represents mean ± SEM of minimum three independent experiments and * Denotes significant difference (P < 0.05) as compared to control (N)

Article Snippet: Ltd; Cat. No. GX-8701M), anti-HIF-1α (Rabbit monoclonal antibody by Invitrogen Cat. No. 700505) and anti-VEGF (Rabbit polyclonal antibody from Puregene by Genetix Biotech Asia Pvt.

Techniques: Expressing, Amplification, Concentration Assay, Reverse Transcription Polymerase Chain Reaction

Journal: Biological Research

Article Title: CoCl 2 simulated hypoxia induce cell proliferation and alter the expression pattern of hypoxia associated genes involved in angiogenesis and apoptosis

doi: 10.1186/s40659-019-0221-z

Figure Lengend Snippet: Western blot analysis of β -actin, HIF-1α, VEGF, p53 and BAX protein in breast cancer cells grown under hypoxia (treated with CoCl 2 ) as well as in normoxia (without treatment). a Differential expression pattern of HIF-1α and VEGF protein isolated from MCF-7 cells. b Shows the dose dependent enhancement in expression of hypoxia associated proteins HIF-1α, VEGF, p53 and BAX in MDA-MB-231 cells grown under hypoxic condition, P < 0.05

Article Snippet: Ltd; Cat. No. GX-8701M), anti-HIF-1α (Rabbit monoclonal antibody by Invitrogen Cat. No. 700505) and anti-VEGF (Rabbit polyclonal antibody from Puregene by Genetix Biotech Asia Pvt.

Techniques: Western Blot, Expressing, Isolation

Journal: Biological Research

Article Title: CoCl 2 simulated hypoxia induce cell proliferation and alter the expression pattern of hypoxia associated genes involved in angiogenesis and apoptosis

doi: 10.1186/s40659-019-0221-z

Figure Lengend Snippet: Relative fold change in expression of HIF-1α and VEGF in MCF-7 cells treated with CoCl 2 in a dose dependent manner has been analysed in qPCR data a as compare to cells grown under normoxia, where β-actin used as a endogenous control. While b represents the qPCR data of HIF-1α, VEGF, p53 and BAX gene in MDA-MB-231 cells, P < 0.05

Article Snippet: Ltd; Cat. No. GX-8701M), anti-HIF-1α (Rabbit monoclonal antibody by Invitrogen Cat. No. 700505) and anti-VEGF (Rabbit polyclonal antibody from Puregene by Genetix Biotech Asia Pvt.

Techniques: Expressing